Viability of dourado embryos cooled in different cryoprotectant solutions
نویسندگان
چکیده
منابع مشابه
Cryopreservation of common carp (Cyprinus carpio) embryos using different cryoprotectant
This study reports that common carp (Cyprinus carpio) embryos were successfully frozen and preserved by vitrification. The modified Haga solution was tested by using two different cryoprotectants and three different concentrations at 4°C to obtain appropriate vitrified solutions ((dimethylsulfoxide 5, 10, 15% (DMSO) and glycerol 5, 10, 15% (gly)) in the study. The hatching rates of embryos exp...
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Equine embryos are traditionally transported in Ham’s F10, a culture medium that requires special handling (bubbling with a specific gas mixture) before it can be used for the conservation of equine embryos. Two media have been tested for the cooled storage of equine embryos (Emcare Holding Solution and Vigro Holding Plus) and appear to be suitable alternatives to Ham’s F10. These media offer t...
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Freezing of ejaculated and epididymal spermatozoa is currently a subject of interest with the purpose of establishing an efficient gene banking model for valuable animals or endangered species. Therefore, the present study evaluated the influence of different storage duration )0, 1.5 and 5 h) at 5 ºC and type of cryoprotectant on freezability of ram epididymal spermatozoa. With increasing the s...
متن کاملTiming of The First Zygotic Cleavage Affects Post-Vitrification Viability of Murine Embryos Produced In Vivo
Background Timing of the first zygotic cleavage is an accurate predictor of embryo quality. Embryos that cleaved early (EC) have been shown to exhibit higher developmental viability compared to those that cleaved at a later period (LC). However, the viability of EC embryos in comparison to LC embryos after vitrification is unknown. The present study aims to investigate the post-vitrification de...
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In this study the efficacy of the combination of glycerol (GLY) and ethylene glycol (EG) as cryoprotectants in a vitrification method developed for direct embryo transfer was evaluated by in vitro development of in vitro fertilized (IVF) and somatic cell nuclear transfer (SCNT) embryos after vitrification. The IVF and SCNT blastocysts were vitrified in either 40% GLY, 30% GLY + 10% EG, or 20% G...
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ژورنال
عنوان ژورنال: Pesquisa Agropecuária Brasileira
سال: 2013
ISSN: 0100-204X
DOI: 10.1590/s0100-204x2013000800057